Measure the volume and apply the sample:
Count the cells with tails and observe their motility and morphology:
Record your observations with your phone camera and get images like this one from an iPhone 4S.
1. Collect the sample.
2. Wait for the sample to liquefy (0-20 minutes).
3. Measure the sample volume in the graduated measurer. 1.5mL is a reference fertility minimum.
4. Turn on the scope LED light using the switch.
5. Smear a small drop of the sample on the objective lens with the micro-pipette.
6. Observe the sample through the eyepiece.
7. Count the number of cells with tails. 15 in view equals 15 million/mL and is a reference minimum.
8. Note motility characteristics. 50% swimming vigorously and progressively is a reference minimum.
9. Inspect the morphology of the cells. Grading them takes experience, but looking can reveal a lot.
After observing a sample you will probably have lots of questions. A simple internet search will produce more information than we can provide. Wikipedia and WebMD are good starting points for deeper searches. If you have questions about how to use the scope just let us know.
The volume of the liquid you can see in focus when you look into the microscope is shaped like a microscopic wading pool. The volume is about 190 microns in diameter and is about 35 microns deep. This amount of liquid is equal to 1 nanoliter.
One million of these volumes makes 1 milliliter. Therefore the conversion factor is one million when calculating your count. Twenty cells with visible tails in the scope's view is equal to 20 million/mL. Likewise, if you can count 15 cells with tails then you have 15 million/mL.
These images were generated from a computer model to simulate a 22 million /mL count.